The Supreme Court has dismissed the president of the Venezuelan Red Cross Society for the first time in more than 40 years.
Mario Enrique Villarroel has been accused of "harassment and abuse" and is being investigated by prosecutors.
A member of the charity's board of directors was also fired.
In a statement, the Venezuelan Red Cross expressed its "absolute and unconditional support" for Villarroel.
Meanwhile, the court ordered a "massive and multifaceted restructuring" of the charity. Ricardo Cusanno, former chairman of Venezuela's largest economic group Fedécamaras, has been appointed chief executive officer as part of a restructuring.
The court declared that the work of the Venezuelan Red Cross was unimpaired.
The ruling also said it would cooperate with the attorney general's office in prosecuting outgoing executives.
Over the years, Red Cross operations in the country have focused primarily on preventing the humanitarian impact of armed violence.
The charity's work also aims to help prisoners and find solutions to water scarcity and food security in a troubled economy.
International charities have called on President Nicolas Maduro's government not to allow the court to intervene.
Miguel Villarroel, vice president of the International Federation of Red Cross and National Red Crescent Societies, said in a video message on Thursday: It will defile our organization that has lasted for 128 years. "
